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KMID : 0378019690120100041
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New Medical Journal
1969 Volume.12 No. 10 p.41 ~ p.44
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Incorporation Rates of L-Leucine-^(14)C into the Visceral Organs in Burned Rabbits
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Abstract
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Since the Cuthbertson´s report that traumatic injuries cause a marked increase of non-protein nitrogen in urine, it has been generally believed that the traumatic injuries enhance the catabolism of the body, especially of the skeletal muscle, and the increased catabolism is caused by the reaction of the adrenal cortex to the trauma. However, it has also been reported by many investigators that injuries stimulate and increase the anabolism as well as catabolism.
Except Whipple et al.´s report the articles concerning the study of protein Metabolism following injuries refered to each individual organ ef. liver or pancreas, etc.
The authors intented to compare the incorporation rate of L-leucine-U-14C per gram of protein of metabolically important organs in or between a normal and a burned rabbit.
Materials and methods:
L-leucine-U-t4C.(20 uCi/kg of body weight) was injected intravenously into two groups of male rabbits, 4 done group consisting of normal 20 rabbits and the other group 20 burned ones. Both these groups were sacrificed 48 hours after L-leucine-U-14C injection and the organs examined included the parotid gland, z sublingual gland, pancreas, stomach, duodenum, small intestine, colon, liver, kidney, and spleen. The amount of tissue protein was determined by Lowry´s method and the radioactivity of tissue protein was measured by 2nc gas flow proportional counter.
The results were as follows:
1) The high incorporation rate of L-leucine-U-F4C per gram of protein was confirmed in the duodenum, salivary glands, small intestine, pancreas and the parotid gland in order of decreasing grade.
2) In burned rabbits, the pancreas and the spleen showed the largest increased incorporation rate, and on the contrary, the parotid gland showed the highest decreased incorporation rate and the kidney showed no changes at all compared with normal rabbits
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